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KMID : 0848120090340030151
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International Journal of Oral Biology
2009 Volume.34 No. 3 p.151 ~ p.155
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Swelling-activated Channels in Human Salivary Gland Acinar Cel
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Chung Ge-Hoon
Sim Jae-Hyun
Kim Soung-Min
Lee Jong-Ho
Chun Gae-Sig
Choi Se-Young
Park Kyung-Pyo
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Abstract
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The role of channels in regulatory volume decrease (RVD) in human salivary gland acinar cells was examined using a whole-cell patch clamp technique. Human tissues were obtained from healthy volunteers or from patients with oromaxillofacial tumors. During the measurements, -free solutions were employed to eliminate contamination of whole-cell conductance by currents. When the cells were exposed to a 70% hypotonic solution, outward-rectifying currents, which were not observed in the resting state, were found to have significantly increased both in human labial and parotid gland acinar cells. The amplitudes of the currents were reduced in a low bath solution. Furthermore, the addition of 5-Nitro-2- (3-phenyl propylamino) benzoic acid (NPPB) or 4,4’-diisothio cyanatostilbene-2,2’-disulphonic acid (DIDS), known to partially block channels, significantly inhibited these currents. Its outward-rectifying current profile, shift in reversal potential in a low bath solution and pharmacological properties suggest that this is a -independent, volume activated current. We conclude therefore that volume activated channels play a putative role in RVD in human salivary gland acinar cells.
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KEYWORD
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channels, Regulatory volume decrease (RVD), Hypotonic, NPPB, DIDS
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